Research article alloxan induced diabetes mellitus in wistar. Alloxan definition of alloxan by the free dictionary. Diabetes was induced by alloxan monohydrate 150 mgkg, i. Archibald from the hospital of the rockefeller institute for medical research, new york. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. The beta cell toxic action of alloxan is initiated by free radicals formed in this redox reaction.
Sigmaaldrich offers a number of alloxan monohydrate products. Further, it is a strong oxidizing agent and it forms a hemiacetal with its reduced reaction product dialuric acid. Imides are less basic yet and in fact react with strong bases to form salts. Biochemical and haematological effects of the leaf extract of. Organic imides, of which alloxan is one, react with azo and diazo compounds to generate toxic gases. The blood glucose levels was significantly p alloxan induced diabetic rats. Electrochemical reduction of alloxan monohydrate on hmde. The mechanism of alloxan and streptozotocin action in b cells. A sum of fourteen different types of spices at different percentages were taken into consideration to propel the research activities forward, viz. The alloxan molecule is structurally so similar to glucose that comparison of the chemical properties of alloxan and streptozotocin alloxan streptozotocin chemical name 2,4,5,6tetraoxypyrimidine. Studies suggests that alloxan does not cause diabetes in humans. According to articles i found that suggested dose to induce diabetes 1 in albino rats is about 140 mgkg. Pdf estimation of glucose level and body weight in. This compound has a high affinity for water and therefore exists as the monohydrate.
Get details of alloxan monohydrate manufacturers, alloxan monohydrate suppliers, alloxan monohydrate dealers, alloxan monohydrate exporters, alloxan monohydrate traders, alloxan monohydrate producers, alloxan monohydrate wholesalers, alloxan monohydrate companies. Ureide is a compound derived from urea and contains acid radicles. Alloxan diabetes definition of alloxan diabetes by. Flammable gases are formed by the reaction of organic imides with strong reducing agents. The use of nonpharmacological treatments herbal agents is a new approach in the management of diabetes. The main purpose of the study was to reveal the hypoglycemic effect of a combination of some selected spices along with the evaluation of nutritional values. The product is washed while still on the funnel with cold glacial acetic acid until the washings are practically colorless. Alloxan is one of the common diabetogenic agents often used to assess the antidiabetic potential of both pure compounds and plant. Studies on the administration of vernonia amygdalina del. After an overnight fast, the rats were injected single dose 125mgkg of freshly prepared 5% solution of alloxan monohydrate in 0. Alloxan 150 mgkg as a 5% solution in normal saline was given to rabbits via marginal ear vein.
Alloxancatalyzed biomimetic oxidations with hydrogen. Alloxan article about alloxan by the free dictionary. The hyperglycemia was confirmed after 72 hrs by the elevation of blood glucose and the behavioral changes excess thirst and frequent urination. The mechanisms of alloxan and streptozotocininduced diabetes. Alloxan, in the presence of intracellular thiols, generates reactive oxygen species ros in a cyclic reaction with its reduction product, dialuric acid.
Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Since the molecular weights of alloxan monohydrate 160 and violuric acid 157 are nearly identical, for practical purposes each mg. Antidiabetic activity of aqueous extract of cinnamomum. The rats with blood glucose level more than 250 mgdl were used for the study.
The bile content of channa punctatus was administered orally in an aqueous solution at a dose of 5mlkg body wt. Alloxaninduced diabetes, a common model for evaluating. After 24 hours, blood glucose content was measured by using clever check glucose test meter bio land, germany using blood sample collected from the tail vein of the rats. Alloxan or mesoxalylurea is an organic compound based on a pyrimidine heterocyclic skeleton.
Chemical properties of alloxan cas 3237501 download as pdf file download as excel file download as 2d mole file predict properties. Alloxan which is chemically known as 5,5dihydroxyl pyrimidine2,4,6trione is an organic compound, a urea derivative, a carcinogen and cytotoxic glucose analog. Parchem is open and ready to supply materials 18002823982 read more. The alloxan was administered intraperitonealy at a dosage of 100mgkg body weight. Its formal name is 2,4,5,61h,3hpyrimidinetetrone, indicating that it has a pyrimidine ring structure with four carbonyl groups. Alloxan definition is a crystalline compound c4h2n2o4 causing diabetes mellitus when injected into experimental animals. Antidiabetic evaluation of bile content of channa punctatus. The animals were then kept for the next 24 hours on 10% glucose to prevent hypoglycemia. What is the right dose of alloxan to induce diabetes 1 in.
Alloxan monohydrate, alloxan monohydrate supplier, alloxan monohydrate distributor, cas 3237501, alloxan monohydrate manufacturer, alloxan monohydrate wholesale skip to main content covid19 status. Then the reaction slurry, which contains the major amount of alloxan monohydrate in crystalline form, is cooled to 510 and filtered through a 5in. Antidiabetic drugs in combination with hydroxychloroquine. A freshly prepared alloxan monohydrate 65 mgkg in 0. Alloxan definition of alloxan by medical dictionary. Hypoglycemic effect study of a combination of some stipulated. After that hypoglycemia was confirmed by diastrix strips. Research article alloxan induced diabetes mellitus in. Allantoin is an urinary excretion product of purine metabolism in most mammals but not in higher apes including humans. Alloxan monohydrate was used to induce diabetes mellitus. Alloxan monohydrate manufacturers, suppliers, exporters.
Applied doses did not cause any acute toxicity or behavioural changes. Both alloxan and glucose are hydrophilic and do not penetrate the lipid bilayer of the plasma membrane. Blood samples were drawn by retroorbital puncture and plasma glucose levels were estimated by glucose oxidase. Louis, mo, usa was used to induce diabetes in adult wistar rats of both sexes. The study was carried out to evaluate the antidiabetic and hypolipidemic activity of ethanolic extract of seeds of eriobotrya japonica ebjs in alloxan induced diabetic albino rats. Estimation of glucose level and body weight in alloxan induced diabetic rat treated with aqueous extract of garcinia kola seed. It is also used in research models to induce diabetes and destroy beta cells.
Diabetes was induced in animals in groups ce using alloxan monohydrate 5% wv bdh laboratory reagents and chemical limited, poole, england. Alloxan monohydrate supplier distributor cas 3237501. The aim of this study was to investigate the effect of aqueous extract of alfalfa on blood glucose and serum lipids in alloxan induced diabetic rats. Diabetes was induced in albino rats by administration of alloxan monohydrate 120 mgkg by intraperitoneal route. The induction of diabetes was confirmed after the 5th day of alloxan treatment by. Hypoglycemic activity of roots of phyllanthus reticulatus in. After 48 hours of administration, it produced diabetes by selectively destroying cells of islets of langerhans. The compound has the molecular formulae, c 4 h 2 n 2 o 4 and a relative molecular mass of 142. Antidiabetic and haematinic effects of parquetina nigrescens. Alloxan catalyst 5c, as its monohydrate form, was purchased from commercial suppliers. Glu 28 cose solution 50% was used to prevent the initial hypoglycemia caused by alloxan monohydrate 29.
The effects of aqueous extract of alfalfa on blood glucose. Join researchers using high quality alloxan monohydrate. Alloxan is a thermally stable white solid with an unusual structure. When the blood glucose level was tested using the glucose oxidase kit, it was observed that the group of animals administered with a combination of vernonia amygdalina extract and metformin shows significant decrease in the blood. Alloxan induced rats showed a significant increase p alloxan induced diabetic rat treated with aqueous extract of garcinia kola seed. Those from one molecule of urea, as alloxan, are monoureides.
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